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UOY

PhD in Biology - Developing novel insecticidal proteins for crop protection

University of York Department of Biology
Self-funded ⏰ Closing Soon 🎓 Agricultural Sciences 🎓 Biochemistry 🎓 Biotechnology biotechnology molecular biology biochemistry recombinant protein production insecticidal proteins crop protection toxicity testing structural biology

Explore novel insecticidal proteins to develop sustainable crop protection methods. Use molecular biology and toxicity testing techniques while collaborating with industry leaders. Contribute to reducing chemical pesticide reliance by pioneering bio-based alternatives.

AI-generated overview

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Why This Research Matters

The research aims to deliver sustainable crop protection by developing bio-based insecticidal proteins. This reduces the environmental and health risks associated with chemical pesticides and supports global food security through innovative pest control strategies.

Enzymology biochemistry

Project Description

Project Overview

Undertake a cutting-edge PhD research project at the University of York’s prestigious Centre for Novel Agricultural Products (CNAP), focused on one of the most pressing challenges in agriculture: effective and sustainable pest control. You will characterise newly identified insecticidal proteins and evaluate their potential as an environmentally friendly, bio-based solution for protecting crops against major agricultural pests, in collaboration with Syngenta.

What You Will Do

  • Recombinant protein production: Use advanced molecular biology techniques to produce recombinant insecticidal proteins in the lab, using state-of-the-art chromatography techniques.
  • Physico-chemical characterisation: Study purified proteins with advanced physico-chemical methods to determine protein purity, oligomeric state, and structure-function properties.
  • Toxicity testing: Evaluate insecticidal potential through in vivo toxicity assays using model insect species and real agricultural pests, measuring mortality, developmental disruption, and behavioural effects to gain insight into mode of action.

Expected Outcomes

Your research will contribute to the development of sustainable pest control strategies, reducing reliance on chemical insecticides and promoting environmental health. You will work in an interdisciplinary team leveraging CNAP’s world-class facilities and collaborate with Syngenta to enhance the research impact.

Why This Matters

The study promotes environmentally friendly crop protection by exploring bio-based insecticidal proteins. This addresses global agricultural challenges by potentially replacing harmful chemical insecticides, fostering ecological balance and food security.

Entry Requirements

Students with, or expecting to gain, at least an upper second class honours degree, or equivalent. Applications are welcomed from biological, chemical, physical science backgrounds, or mathematical backgrounds interested in biological questions.

How to Apply

Register your interest via the university's inquiry system. Contact: Bibash Dhakal at bibashdhakal2003@gmail.com

Eligibility

UK/Home
EU
International

Supervisor Profile

DF
Dr Federico Sabbadin
University of York, Department of Biology

Dr Federico Sabbadin leads this project at the University of York’s Centre for Novel Agricultural Products. His research focuses on molecular biology and structural elucidation of proteins with applications in agriculture and biotechnology. Dr Sabbadin is known for his expertise in protein biochemistry and developing bio-based solutions to agricultural challenges.

Key Publications

2013 401 citations
Key role for a glutathione transferase in multiple-herbicide resistance in grass weeds
2018 308 citations
An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion
2021 224 citations
Secreted pectin monooxygenases drive plant infection by pathogenic oomycetes
2025 74 citations
Oomycetes manipulate plant innate immunity through galacturonide oxidases
2010 72 citations
LICRED: A versatile drop‐in vector for rapid generation of redox‐self‐sufficient cytochrome P450s

Research Contributions

Identified a key role for glutathione transferase in multiple-herbicide resistance in grass weeds.
Provides insight into combating herbicide resistance in agricultural weeds.
Characterized an ancient family of lytic polysaccharide monooxygenases involved in arthropod development and biomass digestion.
Advances understanding of biomass conversion and arthropod physiology.
Discovered secreted pectin monooxygenases that enable plant infection by pathogenic oomycetes.
Helps develop strategies to protect crops from oomycete infections.
Developed a versatile vector (LICRED) for rapid generation of redox-self-sufficient cytochrome P450 enzymes.
Facilitates engineering enzymes for biocatalysis and bioremediation applications.

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