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Regulation of brain fluid homeostasis by dynamic aquaporin-4 subcellular relocalisation

Aston University College of Health and Life Sciences
Partially Funded 🎓 Nursing & Health aquaporin-4 brain fluid homeostasis neurodegeneration brain oedema biochemical methods biophysical methods gliopathy glymphatic system

Explore how aquaporin-4 localization controls brain water balance using cutting-edge methods. Aim to uncover mechanisms linking water transport regulation to neurodegeneration and brain swelling, offering pathways to new treatments.

AI-generated overview

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Why This Research Matters

Understanding aquaporin-4 regulation is crucial to controlling brain water balance and edema, which are key factors in stroke, traumatic injury, ageing, and neurodegenerative diseases. This research may lead to novel interventions to prevent brain swelling and protect neurological function, addressing significant clinical unmet needs.

Aquaporin-4 Brain Water Homeostasis Neurodegeneration Glymphatic Function Subcellular Relocalisation

Project Description

Project Overview

Aquaporin-4 facilitates bidirectional water movement in brain and spinal cord, controlling cell volume, extracellular space, and astrocyte migration. Its subcellular localization dynamically changes, influencing water permeability. Reduced glymphatic function and altered aquaporin-4 localization occur in ageing, stroke, injury, and sleep disruption, all risk factors for neurodegeneration. Brain and spinal cord oedema result from water influx via aquaporin-4 after insults. Modulating its subcellular relocalisation offers clinical benefits.

What You Will Do

You will use in vitro and in vivo methods alongside cutting-edge biochemical and biophysical approaches to characterize the regulation of aquaporin-4 localization in health and disease.

Expected Outcomes

Identification of mechanistic frameworks explaining brain water homeostasis and how targeting aquaporin-4 relocalization can reduce brain swelling and improve recovery after CNS injuries.

Why This Matters

The project addresses urgent clinical needs by investigating mechanisms that contribute to neurodegeneration and brain oedema, advancing potential treatments to prevent brain swelling and related neurological damage.

Entry Requirements

Candidates should have a First or Upper Second Class undergraduate degree in a relevant subject, or a First or Upper Second Class undergraduate degree plus a Merit or Distinction in a relevant Masters degree. Overseas qualifications are considered equivalent according to university criteria.

How to Apply

Applications must include transcripts, a research statement, personal statement, CV, two academic references, English language evidence, and passport copy. Contact Dr Philip Kitchen at p.kitchen1@aston.ac.uk for enquiries and discuss consumables costs prior to application.

Eligibility

UK/Home
EU
International

Supervisor Profile

DP
Dr Philip Kitchen
Aston University, College of Health and Life Sciences
3732 Citations
26 h-index
Google Scholar

Dr Philip Kitchen researches molecular physiology focusing on aquaporin water channels, especially aquaporin-4 in the central nervous system. His work examines aquaporin-4's dynamic subcellular localization and its roles in brain water homeostasis and oedema after CNS insults. He integrates biochemical, biophysical, and in vivo models to translate findings to potential therapies. Dr Kitchen is a recognized expert in aquaporin biology and neurophysiology.

Key Publications

2020 494 citations
Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema
2014 418 citations
Human aquaporins: regulators of transcellular water flow
2021 297 citations
Emerging roles for dynamic aquaporin-4 subcellular relocalization in CNS water homeostasis
2021 283 citations
THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: Ion channels
2023 254 citations
The Concise Guide to PHARMACOLOGY 2023/24: Ion channels

Research Contributions

Explored the regulation and subcellular localization of Aquaporin-4 in the central nervous system.
This work advances understanding of CNS edema treatment and water homeostasis in the brain.
Detailed the role of human aquaporins in transcellular water flow.
Provides foundational knowledge for targeting aquaporins in medical and pharmacological contexts.
Contributed to pharmacological guides focusing on ion channels, indicating expertise in ion channel function and pharmacology.
Helps guide drug development and research in ion channel pharmacology.

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